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Record W2151306218 · doi:10.1051/fruits:2009021

Protoplast isolation and culture for banana regeneration via somatic embryogenesis

2009· article· en· W2151306218 on OpenAlexaff
R. Haïcour, Akym Assani, Viet BuiTrang, Abdelkarim Guédira

Bibliographic record

VenueFruits · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant tissue culture and regeneration
Canadian institutionsUniversity of Guelph
Fundersnot available
KeywordsProtoplastSomatic embryogenesisMusaceaeRegeneration (biology)BiologyIsolation (microbiology)Somatic cellBotanyTissue cultureEmbryogenesisEmbryoMicrobiologyCell biologyGeneticsIn vitro

Abstract

fetched live from OpenAlex

Introduction. Le protocole decrit une methode qui permet d'obtenir des protoplastes a partir de feuilles, de cals et de suspensions cellulaires de bananiers, ainsi que le developpement, par embryogenese somatique, des protoplastes issus de suspensions cellulaires embryogenes. Le principe, les principaux avantages de la methode, le materiel vegetal de depart, le temps necessaire et les resultats attendus sont presentes. Materiel et methodes. Cette partie decrit le materiel de laboratoire necessaire, la preparation des milieux pour l'obtention de protoplastes, ainsi que leur culture. Resultats. Des la premiere demi-heure d'incubation dans la solution enzymatique, les premiers protoplastes sont liberes. En 12 semaines, les protoplastes, issus de suspensions cellulaires embryogenes, se developpent en plantes entieres via l'embryogenese somatique. Sur les couches nourricieres, les protoplastes reconstituent leur paroi ; les premieres divisions cellulaires s'observent (3 a 8) jours apres l'etalement des protoplastes. La formation des proembryons se deroule entre (14 et 21) jours apres le debut des cultures de protoplastes. Puis, (8 a 10) semaines apres l'etalement des protoplastes, leur evolution embryogene conduit a des plantules qui s'enracinent et s'allongent apres transfert sur un milieu depourvu de facteurs de croissance.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.015

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0050.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.236
Teacher spread0.227 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations8
Published2009
Admission routes1
Has abstractyes

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