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Record W2153248406

Effects of various toxicants on uptake of amino acids and their incorporation into proteins by Tetrahymena pyriformis

2006· article· en· W2153248406 on OpenAlexaboutno aff
Antonella Cella, Levon Fendekian, Didier Gauthier

Bibliographic record

VenueProtistology · 2006
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPhotosynthetic Processes and Mechanisms
Canadian institutionsnot available
Fundersnot available
KeywordsTetrahymena pyriformisAmino acidMethionineChemistryCysteineProtein biosynthesisBiochemistryTetrahymenaEnzyme
DOInot available

Abstract

fetched live from OpenAlex

Dupartement de Chimie et Biochimie, Universitu de Moncton, Canada Summary We have investigated the effect of various inorganic and organic toxicants on uptake of amino acids and their incorporation into proteins by Tetrahymena pyriformis. We have studied some heavy metalsCd, Cu, Zn and Cr (dichromate) and organic compounds (2,4�dinitrophenol, sodium pentachlorophenate, 4�chlorophenol and the pesticide Dicamba™). All these toxicants inhibited both activities. Furthermore, the inhibition patterns of amino acid uptake and protein synthesis were similar. This resemblance was observed with variously labeled amino acids: (35S)�methionine, a mixture of ( 35S)� methionine and (35S)�cysteine or a mixture of ( 14C)�amino acids. The ratios of inhibition of amino acid uptake/incorporation were quite close to one in most cases. Most probably, the toxicants tested inhibited protein synthesis and amino acid uptake indirectly, by affecting some other cell process influencing both uptake and incorporation. These results urge to exercise caution when interpreting data on the effect of toxicants on protein synthesis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.011
Threshold uncertainty score0.453

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.003
GPT teacher head0.193
Teacher spread0.190 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2006
Admission routes1
Has abstractyes

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