Probe the Interaction of Sodium Dodecyl Benzene Sulfonate to Papain on Molecular Level.
Bibliographic record
Abstract
In this paper, the effects of sodium dodecyl benzene sulfonate (SDBS) on the structure of papain were evaluated by the fluorescence spectroscopy and UV-vis absorption spectroscopy. The fluorescence emission spectra and synchronous fluorescence spectra indicated that there were interactions between SDBS and papain. Meanwhile, the microenvironment of the amino acid residues in the papain was changed. The UV-vis absorption results showed appreciable conformational changes in papain. The above results are significant in evaluating the toxic effects induced by SDBS in vivo. In this study, we evaluated the mechanism of inter- action between papain and SDBS by the fluorescence spectroscopy and UV-visible absorption spectroscopy. This study not only explores the impacts of SDBS on the structure and function of papain, but also indicates the threat from SDBS to the human health in vivo. 2. EXPERIMENTAL 2.1. Reagents Papain was purchased from Sigma. Sodium dodecyl benzene sulfonate (SDBS) was produced by Sinopharm Group Co., Ltd. HCl was bought from Tianjin Guangcheng Chemical Reagent Co., Ltd. Silver nitrate and 3-hydroxymethyl-methane (Tris) (Sinopharm Chemical Reagent Co., Ltd.) were used as supplied. All solutions were prepared with ultra pure
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".