Effect of ultrasound on human umbilical cord peri-vascular cells
Bibliographic record
Abstract
Background: Tissue engineering involves using different types of stem cells. One of the roadblocks in tissue engineering is the scant supply of stem cells. The potential use of human umbilical cord peri-vascular Cells (HUCPVCs) has recently been considered as an important cell source for tissue engineering applications. The objective of this study was to explore the effect of low intensity pulsed ultrasound (LIPUS) on HUCPVCs. Materials and methods: HUCPVCs were divided into two groups: treatment group which received 30 mW/cm2 LIPUS for 10 minutes (1, 7, and 14 days) and control group which received sham treatment. The study groups were evaluated for cell count, alkaline phosphatase (ALP) activity, DNA-content, gene expression, and immunophenotype. Results: There was no significant differences in cell count, ALP, DNA-content, and CD-90 between LIPUS and control groups. A significantly higher expression of OSP and PCNA was observed on day 14 in LIPUS treatment group. Conclusion: LIPUS application for 10 minutes per day for 14 days enhanced OSP and PCNA expression without significant increase in cell count of HUCPVCs. Future research may aim at exploring different LIPUS applications (different time and frequency) to optimize HUCPVCs proliferation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".