Stimulation effects of salts on growth in suspension culture of a mangrove plant, Sonneratia alba, compared with another mangrove, Bruguiera sexangula and non-mangrove tobacco BY-2 cells
Bibliographic record
Abstract
Effects of a wide range of concentrations of four salts (NaCl, KCl, MgCl2, CaCl2) on cell proliferation were investigated in suspension culture of a mangrove plant, Sonneratia alba. The results were compared to those obtained with suspension culture of another mangrove species, Bruguiera sexangula and non-mangrove tobacco BY-2 cells. Effects of salts on suspension cells were determined with both large scale (LS) method using a 100 ml culture flask, and/or a small scale (SS) method using a 24-well culture plate. Settled cell volume (SCV), packed cell volume (PCV), wet weight (WW) and dry weight (DW) were measured in LS method and PCV was in SS method. High concentrations of NaCl (25–100 mM) and MgCl2 (25 mM) showed strong stimulatory effects on growth in S. alba suspension culture, and low concentrations of KCl, MgCl2 and CaCl2 (10 mM) also showed similar stimulatory effects. In contrast, a remarkable stimulatory effect was not observed in B. sexangula and tobacco BY-2 cells. Cationic ions are effective for the growth of suspension cells in both mangrove species S. alba and B. sexangula. The SS method using multi-well culture plate and micro-tube measurement method developed in this report, can be applied for analysis of factors for stress tolerance.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".