PWE-077 Altered Epithelial Tight Junction Expression and Elevated Il 6 Levels in Pouchitis
Bibliographic record
Abstract
Introduction Intestinal epithelial barrier function limits the interactions between microbial antigens and the mucosal immune system. In IBD, epithelial barrier function is impaired with altered expression of tight junctions. We aimed to assess epithelial tight junction expression and mucosal cytokines in acute and chronic pouchitis and non-inflamed pouches of patients with ulcerative colitis. Methods Mucosal biospy samples were taken from ulcerative colitis patients with pouchitis (chronic pouchitis n = 9, acute pouchitis n = 4) and those without pouchitis (n = 11). Epithelial cells were isolated from biopsy tissue after incubation with DTT and EDTA. Eptihelial cell expression of ZO-1, claudin 1 and claudin 2 were measured by multicolour flow cytometry. Cytokines were assessed by multiplex ELISA of biopsy supernatants. The t-test was used for statistical analysis. Results In acute pouchitis ZO-1 was elevated compared with both chronic pouchitis and non-pouchitis (p = 0.008), whilst in chronic pouchtis ZO-1 expression was reduced compared with non pouchitis (p = 0.006). Claudin 1 expression was reduced in chronic pouchitis (p = 0.04), but was not significantly reduced in acute pouchitis. In acute pouchitis, claudin 2 expression was elevated (p≤0.001), but was not increased in chronic pouchitis. IL6 levels were elevated in chronic pouchitis compared with non pouchitis patients (p = 0.01). Conclusion Epithelial tight junction expression was altered in pouchitis in association with increased IL6 levels. Increased claudin 2 expression in acute, but not chronic pouchitis may represent early pathological changes in the development of pouch inflammation. In chronic inflammation the tight junction complex was deranged with reduced expression of both claudin 1 and ZO-1. Increased epithelial barrier permeability due to altered tight junction expression may be a critical mechanism in the development and perpetuation of pouch inflammation. Disclosure of Interest None Declared.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".