264 PORCINE SPERM-HEAD RECEPTOR INTERACTION WITH PROTEINS PERIPHERALLY BOUND TO THE OVIDUCTAL LUMEN
Bibliographic record
Abstract
Here we show that cell–cell interaction between boar spermatozoa and the oviductal lumen surface are mediated by specific receptor–ligand binding. We have previously demonstrated increased sperm viability following incubation of boar spermatozoa with apical plasma membrane (APM) proteins from sow oviductal epithelial cells (Fazeli A et al. 2003 Reproduction 123, 509–517). Fresh intact oviducts were internally flushed with PBS and filled with Sulfo-NHS-LC-biotin (Pierce Biotechnology, Inc., Rockford, IL, USA) in PBS (pH 8.0). Each end was clamped and incubated for 30 min at RT. Unbound biotin was quenched with 50 mM ammonium chloride for 10 min, and biotinylated soluble APM (sAPM-B) preparations were prepared. Percoll-washed boar spermatozoa (25 × 106 mL-1) were incubated with sAPM-B (150 µg mL-1) for 40 min at 39°C in 5% CO2, and unbound sAPM-B was removed. The sperm pellet was resuspended in 0.5% SDS and incubated for 90 min at RT. Solubilized proteins were isolated by centrifugation at 14,000g for 5 min. The proteins were separated by SDS-PAGE alongside non-biotinylated APM and untreated “sperm-only” samples. Biotinylation was detected by NeutrAvidin/HRP. In addition, sAPM-B treated spermatozoa were smeared onto slides for the detection of biotinyl groups by anti-biotin/Alexa Fluor (Molecular Probes, Leiden, NL). NeutrAvidin/HRP Pierce detected a biotinylated sAPM band migrating to approximately 100 kDa in the sperm/sAPM-B sample. This band was not present in the “sperm-only” sample. Detection of in situ-labelled sAPM-B on spermatozoa showed that fluorescence was specific to the sperm head. We conclude that an oviductal protein of approximately 100 kDa is a potential viability-enhancing ligand for a sperm receptor that is mainly located over the acrosomal region. This study was funded by the BBSRC (UK).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".