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Record W2159652965 · doi:10.1002/prot.21016

Solution structure of acyl carrier protein from <i>Nitrosomonas europaea</i>

2006· article· en· W2159652965 on OpenAlexafffundabout
Sampath Srisailam, Jonathan A. Lukin, Adelinda Yee, Anthony Semesi, C.H. Arrowsmith

Bibliographic record

VenueProteins Structure Function and Bioinformatics · 2006
Typearticle
Languageen
FieldMedicine
TopicMicrobial Natural Products and Biosynthesis
Canadian institutionsUniversity Health NetworkStructural Genomics ConsortiumUniversity of Toronto
FundersNational Institute of General Medical SciencesCanadian Institutes of Health ResearchNational Institutes of HealthUniversity of TorontoGenome Canada
KeywordsNitrosomonas europaeaNitrosomonasChemistryOrganic chemistryNitrification

Abstract

fetched live from OpenAlex

Here, we report the solution structure of Q82SY3 from Nitrosomonas europaea. N. europaea is a Gram-negative bacterium with the interesting capability to derive all its energy from oxidation of ammonia to nitrite. It lives in several places such as soil, sewage, fresh water, walls of buildings, and on the surface of monuments, especially in polluted areas where air contains high levels of nitrogen compounds. N. europaea and other ammonia-oxidizing bacteria play a very crucial role in the biogeochemical nitrogen fixing cycle, that is, biological conversion of reduced nitrogen in the form of ammonia or ammonium to oxidized nitrogen in the form of nitrite, nitrate, or gaseous forms (NO, N 2 O), which in turn, enhances nitrogen availability to plants and waste water 1 treatment. We believe Q82SY3 is likely to be an acyl carrier protein (ACP) based on its structural similarity with such proteins from other organisms such as Escherichia coli and the presence of a conserved "DS" motif. In Figure ACP is a small protein family involved in fatty acid biosynthesis. ACP is a cytosolic transport protein that carries fatty acid via thioester linkage facilitated by the conserved serine residue for chain elongation by fatty acid synthase system. In addition to its function as a carrier protein, ACP could also modulate activities of the enzymatic system involved in fatty acid synthesis. Evidence suggests that substituting ACP from one organism to another yields significant variation in the fatty acid distribution. Involvement of ACPs in fatty acid biosynthesis and antibiotic biosynthesis warrants the study of the structure-function relationships of this class of proteins.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.006
Threshold uncertainty score0.012

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0010.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.005
GPT teacher head0.187
Teacher spread0.182 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations4
Published2006
Admission routes3
Has abstractyes

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