1140918040 Prevention of abortion in the CBAxDBA/2 model by intravaginal TGF‐β is associated with local recruitment of predominantly CD8+ Foxp3+ T cells to the genital tract
Bibliographic record
Abstract
Problem: Spontaneous abortions in DBA/2‐mated CBA/J mice can be prevented by an immune response to BALB/c, and CD4 + 25 + Treg cells as well as CD8 + T cells have been proposed to confer protection. Recently a 2 ng dose of intravaginal TGF‐β3 at the time of exposure to DBA/2 semen was shown to be effective. TGF‐β is known to facilitate development of Treg cells. Is there evidence for local Treg induction? Methods: The phenotype of cellular recruitment to the vaginal wall and uterus was established by immunostaining tissue sections from CBA/J females following intravaginal TGF‐β treatment. The phenotype of cells in vaginal washings 48 hr after TGF‐β was determined by flow cytometry. Results: Increased numbers of CD3 + , CD25 + , and CD11c + cells were found in vaginal mucosa with increasing doses of TGF‐β. A 2 ng TGF‐β3 treatment at the time of estrus recruited Foxp3 + cells to the vaginal lumen, and the majority of these were CD8 + ; CD4 + cells were also present, but expressed only low levels of CD25 and CTLA4. A 20 ng dose recruited predominantly CD4 + 8 + Foxp3 + cells. Conclusion: Induction of Tregs to semen‐associated DBA/2 antigens may prevent pregnancy loss in the CBAxDBA/2 model without the need for BALB/c as an immunogen. The Treg phenotype in the genital tract is compatible with additional members of the Treg family that recognize Class I MHC and associated paternal peptides and prevent abortions.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".