Molecular polymorphism and virulence phenotypes of the wheat leaf rust fungus <i>Puccinia triticina</i> in Canada
Bibliographic record
Abstract
Sixty-nine Puccinia triticina isolates from the eastern region of Ontario and Quebec, the prairie region of Manitoba and Saskatchewan, and Alberta were tested for virulence to 22 near-isogenic Thatcher wheat lines in seedling tests and the Thatcher line with Lr22b in adult plant tests. The isolates were also tested for amplified fragment length polymorphism (AFLP) using 10 specific amplification primer pairs. Thirty-seven distinct virulence phenotypes were identified using the Thatcher lines and 69 molecular phenotypes were identified with 164 AFLP markers. The correlation of isolate grouping based on virulence phenotypes and AFLP phenotypes was 0.53. Almost all isolates from Manitoba and Saskatchewan with virulence to Lr17 had AFLP phenotypes that differed significantly from isolates in the same region that were avirulent to Lr17. This indicated that isolates with virulence to Lr17 are most likely a recent introduction to the prairie region. The presence of distinct groups of isolates based on virulence and AFLP variation provides evidence that a number of different P. triticina phenotypes have been introduced to North America.Key words: Puccinia recondita f.sp. tritici, AFLP, specific virulence.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".