Influence of extract from shoots of Taxus baccata var. 'Elegantissima' on mitotic activity of meristematic cells of Allium cepa L. roots
Bibliographic record
Abstract
We investigated the influence of different concentrations of alcoholic extract from young shoots of Taxus baccata var. 'Elegantissima' (TbE), containing paclitaxel (81.6 µg/g fresh mass), on mitotic activity and DNA content in meristematic ` cells of Allium cepn adventitious root tips. During 24 hours of incubation we obtained different level of inhibition of mitotic activity: ranging from slight changes (dilution 1:32), through complete inhibition reversible in postincubation (dilution 1:8), to whole inhibition irreversible in postincubation (dilution 1:4). Inhibition of mitotic activity was connected with an increase of prophases and metaphases and changes in morphology of chromosomes (thick chromosomes of prophases with diluted karyolymph and c-metaphases). During the experiment, the number of aberrations and micronuclei didn't increase but the number of cells with replicated DNA (4C) blocked in G2 phase was higher. This study was done by a flow cytometry method. The optimal concentration of the TbE extract, which inhibited mitotic activity, did not damage the genetic material (chromosomes) visibly.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".