Production and Immobilization of Halophilic Invertase Produced from Honey Isolate Aspergillus niger EM77 (KF774181)
Bibliographic record
Abstract
Honey isolate Aspergillus niger EM77 was a good halophilic invertase producer in the presence of wheat bran as a complete medium (114.55 U/g), using solid state fermentation technique. Different parameters influence the enzyme productivity such as different pH values, temperature, incubation period, nitrogen and carbon sources were investigated. The optimum pH, temperature and incubation period for enzyme production were 5.5, 30 o C and 72 hrs, respectively. Sucrose at 2 % was more suitable carbon source for invertase production (144.39 U/g) and (NH4)2SO4 at 0.15 % was the ideal nitrogen source. Among different metals ions MnSO4 enhanced the enzyme productivity than other tested ions to 194.71 U/g. The partially purified enzyme was successfully entrapped in polyvinyl alcohol sponge shielded with agar starch layer (PVAsp Gs) and achieved 71% immobilization yield. The optimum conditions for immobilization were: pH 5.2, an incubation time of 15 min and a protein concentration of 250 mg/ml. Immobilized enzyme was reused 12 times with 29% activity loss. The free enzyme lost its activity completely at 70C after 45 minute and the immobilized form retained 80% of its activity at the same condition.The free and immobilized form reported extreme halophilic property since the highest enzyme activity was obtained between 3.5-5 M.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.005 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".