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Record W2169095038 · doi:10.1101/gr.095976.109

The completion of the Mammalian Gene Collection (MGC)

2009· article· en· W2169095038 on OpenAlexaff
Gary Temple, Daniela S. Gerhard, Rebekah S. Rasooly, Elise A. Feingold, Peter J. Good, Allison Mandich, Jeffrey G. Derge, Jeanne Lewis, Debonny Shoaf, Francis S. Collins, Wonhee Jang, Lukas Wagner, Carolyn M. Shenmen, Leonie Misquitta, Carl F. Schaefer, Kenneth H. Buetow, Tom I. Bonner, Linda Yankie, Ming Ward, Lon Phan, Alex Astashyn, Garth Brown, Catherine M. Farrell, Jennifer Hart, Melissa Landrum, B. Maidak, Michael R. Murphy, Terence D. Murphy, Bhanu Rajput, Lillian D. Riddick, David Webb, Janet A. Weber, Wendy Wu, Kim D. Pruitt, Donna Maglott, Adam Siepel, Broňa Brejová, Mark Diekhans, Rachel Harte, Robert Baertsch, Jim Kent, David Haussler, Michael R. Brent, Laura Langton, Charles L.G. Comstock, Michael Stevens, Chaochun Wei, Marijke J. van Baren, Kourosh Salehi‐Ashtiani, Ryan R. Murray, Lila Ghamsari, Elizabeth Mello, Chenwei Lin, Christa Pennacchio, Kirsten Schreiber, Nicole Shapiro, Amber Marsh, Elizabeth Pardes, Troy Moore, Anita Lebeau, Mike Muratet, Blake A. Simmons, David Kloske, Stephanie Sieja, James R. Hudson, Praveen Sethupathy, Michael Brownstein, Narayan Bhat, Joseph Lazar, Howard J. Jacob, C. E. Gruber, Mark R. Smith, John D. McPherson, Angela Garcia, Preethi H. Gunaratne, Jiaqian Wu, Donna M. Muzny, Richard A. Gibbs, Alice Young, Gerard G. Bouffard, Robert W. Blakesley, Jim Mullikin, Eric D. Green, Mark Dickson, Álex Rodríguez, Jane Grimwood, Jeremy Schmutz, R Myers, Martin Hirst, Thomas Zeng, Kane Tse, Michelle Moksa, Merinda Deng, Kevin Ma, Diana Mah, Johnson Pang, Greg Taylor, Eric Chuah, Athena Deng, Keith Fichter, Anne Go, Stephanie J. Lee, Jing Wang, Malachi Griffith, Ryan D. Morin, Richard A. Moore, Michael Mayo, Sarah Munro, Susan Wagner, Steven J.M. Jones, Robert A. Holt, Marco A. Marra, Lu Sun, Shuwei Yang, James Hartigan, Marcus Graf, Ralf Wagner, Stanley Letovksy, Jacqueline C. Pulido, Keith Robison, Dominic Esposito, James L. Hartley, Vanessa Wall, Ralph F. Hopkins, Osamu Ohara, Stefan Wiemann

Bibliographic record

VenueGenome Research · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMolecular Biology Techniques and Applications
Canadian institutionsBC Cancer Agency
FundersNational Cancer InstituteNational Human Genome Research InstituteU.S. Public Health ServiceNational Institutes of Health
KeywordsRefSeqBiologyGeneGeneticsCloning (programming)Coding regionLibraryComplementary DNAAccession number (library science)EnsemblDNA sequencingExpressed sequence tagcDNA libraryComputational biologyGenBankGenomeGenomics

Abstract

fetched live from OpenAlex

Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.004
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.025
Threshold uncertainty score0.084

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0040.003
Meta-epidemiology (narrow)0.0020.001
Meta-epidemiology (broad)0.0030.002
Bibliometrics0.0040.006
Science and technology studies0.0020.001
Scholarly communication0.0030.001
Open science0.0040.003
Research integrity0.0020.003
Insufficient payload (model declined to judge)0.0250.054

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.035
GPT teacher head0.352
Teacher spread0.317 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations147
Published2009
Admission routes1
Has abstractyes

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