Glucose responsive neurons in the nucleus of the solitary tract are responsive to nesfatin‐1 (1126.3)
Bibliographic record
Abstract
Nesfatin‐1 (nes‐1) exerts important effects on food intake and glucose handling and is expressed in the nucleus of the solitary tract (NTS), a critical medullary autonomic control center. Since hypoglycemia induces cFos expression in nes‐1 immunoreactive NTS neurons, here we examined effects of nes‐1 on the excitability of glucose responsive NTS cells using whole‐cell patch clamp recordings. A total of 44% of neurons tested (7/16) responded to a decrease from 3 to 0.2 mM glucose, 4 with a depolarization (glucose inhibited (GI), mean 6.1 ± 1.6 mV), and 3 with a hyperpolarization (glucose excited (GE), mean ‐5.7 ± 1.4 mV). Bath applied nes‐1 (10 nM) influenced the excitability of all but one of these glucose responsive neurons. All GI neurons responded to nes‐1, 3 with a depolarization (mean 7.6 ± 2.4 mV) and 1 with a hyperpolarization (‐8.5 mV). Moreover, 2/3 GE cells were depolarized by nes‐1 (mean 3.3 ± 1.0 mV) while 1 did not respond. NTS neurons which did not respond to changes in glucose (9/16) were also nes‐1 responsive, with 5 showing a depolarization (mean 3.5 ± 0.4 mV) and 4 a hyperpolarization (mean ‐5.8 ± 0.5 mV). While our findings reveal clear actions of nes‐1 on glucose sensing NTS neurons, they do not suggest functional differentiation between the effects of nes‐1 on GI and GE neurons. Our observations lend further support to the hypothesis that nes‐1 may act as a locally released modulator of intrinsic neuronal function. Grant Funding Source : NSERC, FQRNT, HSFO
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".