Evidence for facilitated uptake of Hg(II) by <i>Vibrio anguillarum</i> and <i>Escherichia coli</i> under anaerobic and aerobic conditions
Bibliographic record
Abstract
A mer‐lux bioreporter was used to study uptake of inorganic mercury, Hg(II), at trace concentrations by two facultatively anaerobic bacterial species, Vibrio anguillarum and Escherichia coli . Uptake of Hg(II) by these bacteria appeared to be facilitated, rather than by passive diffusion. Three lines of evidence support this conclusion. First, under anaerobic conditions Hg(II) uptake was greatly decreased compared with aerobic conditions, even though the chemical composition of the medium was identical except for the lack of oxygen (i.e., no reducing agents were used). Second, the uptake of Hg(II) under anaerobic conditions was not proportional to the abundance of lipophilic Hg species but was dependent on the total concentration of Hg in the samples. Third, at trace Hg(II) concentrations and under anaerobic conditions, Hg(II) uptake was enhanced by the addition of yeast extract and a variety of low molecular weight organic acids. In addition to demonstrating that Hg(II) uptake by these bacteria had the characteristics of facilitated transport, these lines of evidence also support the conclusion that processes under regulatory control of the cell affected Hg(II) uptake. If these findings apply to other bacteria as well, they mean that current models of Hg(II) uptake by microorganisms in aquatic systems, which are based solely on the role of lipophilic Hg species and passive diffusion, will need to be reconsidered.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".