MétaCan
Menu
Back to cohort
Record W2171686328

Model Systems to Explore CPB2 Pre-mRNA Splicing

2014· article· en· W2171686328 on OpenAlexaff
Christina Marisa Rizzo

Bibliographic record

VenueScholarship at UWindsor (University of Windsor) · 2014
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCancer-related gene regulation
Canadian institutionsUniversity of Windsor
Fundersnot available
KeywordsRNA splicingMessenger RNAChemistryComputational biologyComputer scienceBiologyGeneticsGeneRNA
DOInot available

Abstract

fetched live from OpenAlex

Thrombin Activatable Fibrinolysis Inhibitor (TAFI), encoded by CPB2, cleaves carboxyl-terminal lysine residues from partially degraded fibrin, thus assisting in the attenuation of fibrinolysis. The finding of several new alternatively spliced (AS) CPB2 variants within numerous cell types suggests that AS is an important mechanism regulating CPB2 gene expression. This thesis investigated the extent of AS events occurring in various vascular and immune cells, and identified proteins translated from AS CPB2 mRNA. To determine whether a minigene approach could be used to model the cell-specific AS pattern that occurs endogenously, two minigene constructs were created. Real time RT-PCR analysis revealed that the pattern and extent of AS varies in a cell-specific manner. However, the minigenes were found to be unable to recapitulate the cell-specific splicing pattern of CPB2 that occurs endogenously. Metabolic labeling in conjunction with immunoprecipitation resulted in the detection of the Delta7 TAFI variant retained within HepG2 cells.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.395
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.026
GPT teacher head0.237
Teacher spread0.211 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes1
Has abstractyes

Explore more

Same venueScholarship at UWindsor (University of Windsor)Same topicCancer-related gene regulationFrench-language works237,207