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Genomic Regulation through RNA in Oocyte Maturation of Large Mammals

2011· book-chapter· en· W2174535278 on OpenAlexaff
Marc‐André Sirard

Bibliographic record

VenueBENTHAM SCIENCE PUBLISHERS eBooks · 2011
Typebook-chapter
Languageen
FieldMedicine
TopicReproductive Biology and Fertility
Canadian institutionsUniversité Laval
Fundersnot available
KeywordsOocyteBiologyGeneticsRNARna processingEvolutionary biologyComputational biologyGeneEmbryo

Abstract

fetched live from OpenAlex

The oocyte is a unique cell amongst the 212 cell types that makes an individual. This cell does not divide until it resumes meiosis and remains in a special chromatin status during the dictiate stage of the prophase from oocyte formation in the gonad in the female fetus until it dies through apoptosis or proceeds to ovulation. This status requires unique features to allow transcription to be active during oocyte growth and an automatic pilot system to drive the transition from tetraploidy to haploidy during maturation and fertilization and back to diploidy, all this in a few days in large mammals. Therefore, the regulatory program for all the transformations required for chromosome separation, cell cycle progression, response to sperm entry, and embryonic genome activation must be stored in the oocyte prior to ovarian release or even prior to final chromatin condensation as it inhibits further transcription. The data related to gene regulation during this period is limited for two main reasons: limited amount of material to study in mammals and differences with somatic tissues where gene pathways are much better characterized. Nevertheless, using the genomic amplification approaches and the increasing amount of information in somatic tissues and in oocytes from lower species, it is becoming possible to study this automatic pilot system that drives the mammalian oocyte through maturation-fertilization and embryonic genome activation. This chapter will focus on the progression of our understanding of the oocyte using proteomic and transcriptomic tools.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Other · Consensus signal: none
Teacher disagreement score0.809
Threshold uncertainty score0.923

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.001
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.042
GPT teacher head0.278
Teacher spread0.236 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreOther

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2011
Admission routes1
Has abstractyes

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