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Record W2175693398 · doi:10.1073/pnas.1506226112

Genome of <i>Rhodnius prolixus</i> , an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

2015· article· en· W2175693398 on OpenAlexaff
Rafael D. Mesquita, Raquel J. Vionette-Amaral, Carl Lowenberger, Rolando Rivera‐Pomar, Fernando A. Monteiro, Patrick Minx, John Spieth, Antonio Bernardo Carvalho, Francisco Panzera, Daniel Lawson, André Torres, José M. C. Ribeiro, Marcos Henrique Ferreira Sorgine, Robert M. Waterhouse, Michael J. Montague, Fernando Abad‐Franch, Michele Alves‐Bezerra, Laurence Rodrigues do Amaral, Helena Araujo, Ricardo N. Araújo, L. Aravind, Geórgia C. Atella, Patrı́cia Azambuja, Mateus Berni, Paula Bittencourt‐Cunha, Glória Regina Cardoso Braz, Gustavo M. Calderón‐Fernández, Cláudia M. A. Carareto, Mikkel Christensen, Igor Costa, Samara Costa, Marílvia Dansa, Carlos R. O. Daumas-Filho, Iron F. De-Paula, Felipe A. Dias, George Dimopoulos, Scott Emrich, Natalia Esponda-Behrens, Patrı́cia Fampa, Rita D Fernández-Medina, Rodrigo Nunes da Fonseca, Marcio Fontenele, Catrina C. Fronick, Lucinda A. Fulton, Ana Caroline P. Gandara, Elói S. Garcia, Fernando Ariel Genta, Gloria I. Giraldo-Calderón, Bruno Gomes, Kátia C. Gondim, Adriana Granzotto, Alessandra A. Guarneri, Roderic Guigó, Myriam Harry, Daniel S. T. Hughes, Willy Jablonka, Emmanuelle Jacquin‐Joly, M. Patricia Juárez, Leonardo Barbosa Koerich, Angela B. Lange, José Manuel Latorre-Estivalis, Andrés Lavore, Gena G. Lawrence, Cristiano Lazoski, Cláudio R. Lazzari, Raphael R.S. Lopes, Marcelo Gustavo Lorenzo, Magda Delorence Lugon, David Majerowicz, Paula L. Marcet, Marco Mariotti, Hatisaburo Masuda, Karyn Mégy, Ana Claudia A. Melo, Fanis Missirlis, Theo Mota, Fernando G. Noriega, Marcela Nouzová, Rodrigo Dutra Nunes, Raquel L. L. Oliveira, Gilbert O. Silveira, Sheila Ons, Ian Orchard, Lucía Pagola, Gabriela O. Paiva‐Silva, Agustina Pascual, Márcio G. Pavan, Nicolás Pedríni, Alexandre A. Peixoto, Marcos H. Pereira, Andrew Pike, Carla Polycarpo, Francisco Prosdocimi, Rodrigo Ribeiro‐Rodrigues, Hugh M. Robertson, Ana Paula Salerno, Didier Salmon, Didac Santesmasses, Renata Schama, Eloy S. Seabra-Junior, Lívia Silva-Cardoso, Mário A.C. Silva-Neto, Matheus de Souza Gomes, Marcos Sterkel, Mabel L. Taracena, Marta Tojo, Zhijian Tu, José M. C. Tubío, Raùl Ursic-Bedoya, Thiago M. Venâncio, Ana Beatriz Walter‐Nuno, Derek A. Wilson, Wesley C. Warren, Richard K. Wilson, Erwin Huebner, Ellen M. Dotson, Pedro L. Oliveira

Bibliographic record

VenueProceedings of the National Academy of Sciences · 2015
Typearticle
Languageen
FieldMedicine
TopicTrypanosoma species research and implications
Canadian institutionsUniversity of ManitobaUniversity of TorontoSimon Fraser University
FundersNational Institute of Allergy and Infectious DiseasesNational Human Genome Research InstituteU.S. Public Health ServiceNational Institutes of HealthFundación Bunge y BornFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroConselho Nacional de Desenvolvimento Científico e TecnológicoFundação de Amparo à Pesquisa do Estado de Minas GeraisConsejo Nacional de Investigaciones Científicas y TécnicasCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
KeywordsRhodnius prolixusChagas diseaseRhodniusBiologyInsectVector (molecular biology)Parasite hostingVirologyGenomeZoologyTriatominaeGeneticsGeneHemipteraEcologyReduviidae

Abstract

fetched live from OpenAlex

Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (∼ 702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immune-deficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.824
Threshold uncertainty score0.202

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.118
GPT teacher head0.375
Teacher spread0.257 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations363
Published2015
Admission routes1
Has abstractyes

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