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Record W2181886091 · doi:10.1128/9781555816841.ch28

Redox Mechanisms and Reactive Oxygen Species in Antibiotic Action and Resistance

2014· book-chapter· en· W2181886091 on OpenAlex
Inas J. Radhi, Gerard D. Wright

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueASM Press eBooks · 2014
Typebook-chapter
Languageen
FieldEnvironmental Science
TopicPharmaceutical and Antibiotic Environmental Impacts
Canadian institutionsMcMaster University
Fundersnot available
KeywordsReactive oxygen speciesChemistryBiochemistryMicrobiologyAntibioticsRadicalBiology

Abstract

fetched live from OpenAlex

The majority of antibiotics in clinical and agricultural use are derived from secondary metabolites of bacteria, fungi, and plants. This chapter discusses the roles of redox chemical biology in antibiotic action and resistance. Bleomycin is a glycopeptide anticancer antibiotic produced by Streptomyces verticillus. Antimicrobial toxicity is dependent on the reduction of the nitro group via electron transport components, generating a nitro anion radical and breakdown compounds such as nitroso and hydroxylamine derivatives. The nitro anion radicals generated through the reduction by ferredoxin or flavodoxin, may oxidize from the presence of molecular oxygen. Bacterial killing was attenuated by the addition of the iron chelator 2,2'-dipyridyl and the radical scavenger thiourea. The physiological state of microbes also has an important role in reactive oxygen species (ROS) and antibiotic action. Pseudomonas aeruginosa in liquid culture was more susceptible to oxidative stress generated by the antibiotics ceftazidime or piperacillin than during biofilm growth. MexR is a stable homodimer and functions as a transcriptional repressor and is a member of the MarA family transcriptional regulators. Redox and ROS have long been underexplored in antibiotic action and resistance but studies over the past decade have unequivocally demonstrated their importance in this field.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.722
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.040
GPT teacher head0.256
Teacher spread0.217 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it