Bibliographic record
Abstract
RescueMu is a modified Mu1 transposon transformed into maize to permit mutagenesis and subsequent recovery of mutant alleles by plasmid rescue. RescueMu elements insert late in the germline as well as in terminally dividing somatic (e.g., leaf) cells. Germinal insertions may result in a mutant phenotype, and RescueMu permits recovery of 5-25 kb of transposon-flanking genomic DNA without having to construct and screen genomic DNA libraries. Late somatic insertions of RescueMu do not result in a visible phenotype, but they are instead used to construct plasmid libraries of gene-enriched maize genomic DNA to facilitate the identification and sequencing of the euchromatic portion of the maize genome. This is because maize leaves contain abundant independent RescueMu somatic insertions, and 70-90% of these insertions occur preferentially into genes and not repetitive DNA. This chapter describes detailed protocols on how to obtain, generate, and use RescueMu for maize genomics, including resources developed by the Maize Gene Discovery Project (MGDP) consortium available online at ZmDB.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".