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Record W2188538605

Genetic identification of eleven aquatic bacteria using the 16S rDNA gene

2012· article· en· W2188538605 on OpenAlexaboutno aff
Milena Block, Anthony J. A. Ouellette

Bibliographic record

Venuenot available
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsnot available
Fundersnot available
KeywordsBiologyGenBankBacteria16S ribosomal RNAEnterobacterPhylogenetic treeAeromonasMicrobiologyAcinetobacterEcologyZoologyGeneGeneticsEscherichia coli
DOInot available

Abstract

fetched live from OpenAlex

Bacteria help maintain ecological balance by participating in the carbon, oxygen, and/or nitrogen cycles. These cycles are important for the organism’s survival, that’s why their identification is fundamental in order to determine how they function and interact in an ecosystem. In this study, eleven previously isolated bacteria from the upstate New York waterbodies (the Oswego River, Lake Ontario, and Lake Neatahwanta) were identified by sequencing a fragment of the 16S rDNA gene from each bacteria. Identification was supported by metabolic tests. The sequences were compared in the nucleotide database in GenBank (GenBank, 2009) and then aligned to construct a phylogenetic tree. The eleven bacteria were grouped into eight genera: Acinetobacter, Aeromonas, Planococcus, Enterobacter, Exiguobacterium, Pseudomonas, Plesiomonas, and Staphylococcus. The metabolic tests better supported the identification for the samples with longer sequences than samples with shorter sequences, which shows the importance of getting longer sequences to better identify bacteria. The identified genera of the isolated bacteria were found to occur in aquatic environments and Plesiomona shigelloides, one of the samples, is usually found in fish and other aquatic animals. Genetic identification of bacteria 3

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.014
Threshold uncertainty score0.027

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.021
GPT teacher head0.254
Teacher spread0.233 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2012
Admission routes1
Has abstractyes

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