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Record W2189264915

Neuronal repopulation into arcuate nucleus of hypothalamus

2009· article· es· W2189264915 on OpenAlexaff
Francisco Rivera Silva, aEric Avila Orozco, José Luis Pontijas Calderón, Tania Valenzuela, Louis Gagné Vogt

Bibliographic record

VenueArchivos de neurociencias (México, D.F.) · 2009
Typearticle
Languagees
FieldNeuroscience
TopicNeurogenesis and neuroplasticity mechanisms
Canadian institutionsUniversité Laval
Fundersnot available
KeywordsNeuNNeurogenesisArcuate nucleusHypothalamusProliferation MarkerNestinImmunocytochemistryBromodeoxyuridineInternal medicineGlutamate receptorFibroblast growth factorEndocrinologyCell biologyBiologyNucleusChemistryNeural stem cellImmunohistochemistryMedicineStem cell
DOInot available

Abstract

fetched live from OpenAlex

Recent evidences suggest that neurogenesis could occur in different zones of the central nervous system. Objective: with the purpose of analyzing neurogenesis, we have done a study in the arcuate nucleus of hypothalamus after administration of glutamate and epidermal growth factor (EGF) plus fibroblast growth factor-2 (FGF-2). Material and Methods: we have used the electron and light microscopy, immunocytochemistry and immunoflourescence studies and the organotypic cultures. The markers used were NeuN, neurogenin, Tuj 1, CD24, lycopersicum sculetum, DARPP32, vimentin, nestin, PSA-NCAM, caspase 9, BrdU and 3HT. We used rats (P4), the time of sacrifice was at 3 h, 6 h, 12 h, 36 h, 15 d, 30 d, 90 d and 120 d. This research has been divided in 3 experiments. Experiment A, we administrated BrdU (100 mg/kg) and we detected the cellular proliferation. Experiment B, we used glutamate (4 mg/g, sc), and we observed the cellular death. Experiment C, we used glutamate, BrdU and EGF+FGF-2 (5 ng/g, sc). Results and conclusions: the results obtained indicate that we can induce the neuronal regeneration of the arcuate nucleus by means of use of EGF and FGF-2, and we identify the third ventricle like a zone of neurogenesis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.027
GPT teacher head0.279
Teacher spread0.252 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2009
Admission routes1
Has abstractyes

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