Infection and Gene Expression of the Clubroot Pathogen <i>Plasmodiophora brassicae</i> in Resistant and Susceptible Canola Cultivars
Bibliographic record
Abstract
Infection by the clubroot pathogen Plasmodiophora brassicae on resistant and susceptible canola cultivars was investigated at various times following inoculation. Primary infection occurred on more than 90% of root hairs in both cultivars at 7 days after inoculation (dai), and thereafter declined to less than 20% at 14 to 35 dai. The amount of primary infection on the two cultivars was similar at each time point. Secondary infections were rare in both cultivars at 5 and 7 dai but became common after 14 dai. At 14 to 28 dai, the level of secondary infection was greater in the resistant cultivar than in the susceptible one. The in planta expression of 12 selected P. brassicae genes was investigated by reverse-transcription quantitative polymerase chain reaction. All genes were upregulated at 5 or 7 dai in the resistant cultivar. In the susceptible cultivar, the 12 genes could be classified into three groups according to their expression patterns: 2 genes showed an expression peak at 14 dai, 3 showed two expression peaks at 14 and 35 dai, and the others showed an expression peak at 35 dai. Results from this study will be useful in breeding for resistance and in selecting candidate pathogenicity genes for further studies.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".