First evidence of the presence of<i>NK2</i>and<i>Tlx</i>genes in bivalve molluscs
Bibliographic record
Abstract
The NK homeobox gene cluster appears to play a primary role in the mesoderm specification and formation and nervous system development of Bilateria. NK2 class genes are transcribed in the developing gut, nervous system, and heart of insects and vertebrates. Tlx genes are involved in a variety of developmental functions such as neurone differentiation. To identify NK box genes in bivalve molluscs, we performed a polymerase chain reaction (PCR) survey with degenerate primers on genomic DNA. Three NK box sequences were identified from two bivalve species: one from the clam Venerupis pullastra (Montagu, 1803) called Vpu Tlx and two from the oyster Ostrea edulis L., 1758 called Oed Tlx and Oed Nk2. The alignment of these sequences in the UniProt database reveals a high level of conservation. Phylogenetic analyses by NJ, UPGMA, ME, MP, and ML analyses show the orthology relationships of the Tlx and Nk2 genes with other Tlx and Nk2 genes present in Metazoa genomes. This is the first report of the isolation of NK box genes in bivalve molluscs. Moreover these Tlx and Nk2 genes are the first to prove that Tlx and NK2 gene cognates exist in bivalve molluscs. The presence of these genes in Venerupis and Ostrea suggests that these genes could be conserved in bivalves in general.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".