Expression and Imaging of Connexin-GFP Chimeras in Live Mammalian Cells
Bibliographic record
Abstract
Gap junction (GJ) proteins, connexins (Cx), possess many properties that are atypical of other well-characterized integral membrane proteins (). Oligomerization of Cxs into hemichannels (connexons) has been shown to occur after the protein exits the endoplasmic reticulum (). Once delivered to the cell surface, connexons from one cell dock with connexons from a neighboring cell and cluster into GJ plaques, a process that is facilitated by cadherins (). Connexins have short half-lives and GJs are turned over rapidly (). To study GJ formation, removal, and connexin intracellular trafficking in real time, we recently used the green fluorescent protein (GFP) as a fusion partner to construct Cx-GFP chimeras (see Fig. 1). Open image in new window Fig 1. Schematic diagram of the pEGFP vector containing the Cx43 insert. The Cx43 is fused in-frame to the N-terminal domain of GFP.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.002 | 0.002 |
| Insufficient payload (model declined to judge) | 0.004 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".