Molecular Cloning, Characterization, and Expression Analysis of DROOPING LEAF gene in the Pistillody Line of Common Wheat
Bibliographic record
Abstract
In this article, we report the cloning, characterization, and expression pattern of Triticum aestivum DROOPING LEAF gene (TaDL) in pistillody line of common wheat. Three TaDL homoeologous genes (TaDL1, TaDL2, and TaDL3) were isolated and found to be located on chromosomes 4AS, 4DL, and 4BL, respectively. The putative TaDL1, TaDL2, and TaDL3 protein were 22.4, 22.6, and 22.3 kDa, with theoretical pI of 9.04, 9.04, and 8.98 and containing two distinct domains, namely, a zinc-finger domain in the N-terminal region and a C-terminal YABBY domain. The genome sequence of the three homologous genes consisted seven exons and six introns. All the introns, except for intron VI, varied in length and sequence composition. Phylogenetic analysis revealed that TaDL was most closely related to the Brachypodium distachyon BdDL and Oryza sativa DL genes. The expression pattern analysis indicated that TaDL1, TaDL2, and TaDL3 have a higher expression levels in PS and P; however, the three genes are very rare in S. Thus, TaDL gene may contribute to pistil development. Moreover, overexpression of TaDL gene in stamens may cause complete or partial homeosis of stamens into pistils in wheat.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".