Redox modulation of the processing and presentation of myelin-associated antigens in experimental autoimmune encephalomyelitis. (P5184)
Bibliographic record
Abstract
Abstract NOX2 is well known for its microbicidal activity within phagolysosomes of phagocytes. Our research group has recently found that NOX2 activity also impacts two key antigen processing chemistries within the phagosome of macrophages and dendritic cells. NOX2 activation inhibits disulfide reduction and alters proteolysis through the oxidative inactivation of cysteine cathepsins B, S, and L. Both disulfide reduction and proteolysis are vital for antigen and invariant chain processing, and thus are required for efficient antigen processing/presentation. Our objective is to determine if NOX2 influences the progression of EAE, which requires processing of endogenous myelin, by modulating antigen processing and changing the immuno-dominance of MOG peptides. We examined the susceptibility of WT and NOX2-deficient mice to EAE. Varying MOG components were used to induce EAE in WT and NOX2-deficient mice. NOX2-deficient mice were found to be partially clinically protected from MOG35-55 induced EAE, and less so from recombinant MOG induced EAE. Antigen presentation efficiency of MOG35-55 by NOX2-deficient macrophages to 2D2 T cells was reduced compared to WT macrophages when the antigenic source was recombinant MOG, but not when the macrophages were pulsed with MOG35-55. These results suggest that NOX2 may have a role in the modified processing of MOG proteins, which may be partially responsible for the differential susceptibility of NOX2-deficient mice to EAE.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".