New insights into aphid isoprenoid pathway
Bibliographic record
Abstract
FORM Proposal for oral presentation New insights into aphid isoprenoid pathway VANDERMOTEN Sophie, CUSSON Michel, HAUBRUGE Eric, FRANCIS Frederic 1. University of Liege, Gembloux Agro-Bio Tech, Functional and Evolutionary Entomology, Passage des Deportes 2, B-5030 Gembloux, Belgium. 2. Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, P.O. Box 10380, Stn. Ste-Foy, Quebec, QC G1V4C7, Canada. Isoprenoids form an extensive group of natural products involved in a number of important biological processes. In insects, they serve among other as hormones and pheromones. Their biosynthesis proceeds through sequential 1’-4 condensations of isopentenyl diphosphate (C5) with an allylic acceptor, the first of which is dimethylallyl diphosphate (C5). The reactions leading to the production of geranyl diphosphate (GPP; C10), farnesyl diphosphate (FPP; C15) and geranylgeranyl diphosphate (GGPP; C20), which are the precursors of mono-, sesquiand diterpenes, respectively, are catalyzed by a group of highly conserved enzymes known as short-chain isoprenyl diphosphate synthases or prenyltransferases. In recent years, the sequences of many new prenyltransferases have become available, revealing novel mechanisms of product chain-length selectivity and an intricate evolutionary path from a putative common ancestor. We characterized an aphid prenyltransferase producing both GPP and FPP in similar amounts under in vitro conditions. In an effort to identify the mechanism responsible for this dual activity, we assessed the product selectivity of several aphid prenyltransferase site-directed mutants, designed to effect substitutions in amino acid residues seen to be in close proximity to the GPP or FPP ligand in a homology model. Our analyses pointed to two amino acid residues, Gln107 and Leu110, likely to play a key role in conferring dual GPP/FPP synthase activity to the aphid enzyme. Our research is now focusing on the study of the implication of aphid GPP/FPP synthase in juvenile hormone, alarm and sexual pheromones biosynthesis using qRT-PCR and RNAi methods.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".