Regeneration of plantlets of Piper longum L. through in vitro culture from nodal segments
Why this work is in the frame
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Bibliographic record
Abstract
Plantlet regeneration in Piper longum L. has been achieved from nodal segments excised from in vivo grown plantlets cultured on MS medium supplemented with growth regulators. The present investigation was carried out to regenerate plantlet of Piper longum L through in vitro culture. Nodal segments from one year old plants of field grown Piper longum were used as explants for initial culture. The nodal explants were cultured on MS medium supplemented with different concentration and combination of cytokinines and auxines for primary shoot proliferation. The best shoot proliferation was observed in MS medium containing 1.0 mg/l Kinetin and 1.5 mg/l BAP where 98 % of explants showed proliferation with highest rate of shoot multiplication (5-6 shoots per explant). Callus induction occurred in (1 mg/l) BAP and (0.5 mg/l) Kinetin and 10-15 days of callus subculture initiation of greenish white shoot buds was observed. For rooting, the in vitro micro shoot were inoculated to MS basal media supplemented with 0.5 mg/l IAA and rooting was more profuse. The regenerated plantlets were successfully established in soil with survival rate 90%. The protocol described is simple, rapid efficient for in vitro propagation of P. longum (L.) from nodal explants and soil establishment of plantlets.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it