Possible involvement of cytoskeletal reorganization in PKC‐induced cerebroarterial constriction
Bibliographic record
Abstract
Several hypotheses have been proposed to account for force production evoked by PKC activation in the resistance circulation based on pharmacological experiments with intact and permeabilized vessel preparations. These include: an increase in calcium influx, an increase in calcium sensitization via phosphorylation of CPI‐17, and thin filament regulation via phosphorylation of calponin and/or caldesmon. However, biochemical evidence of a role for these phosphoproteins has not been forthcoming. Here, rat middle cerebral arteries were used to investigate the molecular mechanism responsible for PKC‐mediated constriction evoked by 5‐HT or direct activation with phorbol dibutyrate (PDBu). A highly sensitive 3‐step western blotting method was used to detect changes in protein phosphorylation. PDBu caused a much larger contraction compared to that evoked by a maximal concentration of 5‐HT, but a similar increase in LC20 phosphorylation. PKC inhibition with GF109302X abolished the 5‐HT‐induced constriction with minimal change in pLC20 content, indicating a role for a non‐LC20‐dependent mechanism(s). Phosphorylation of calponin and caldesmon were not affected by direct PKC activation. These findings suggest a possible contribution of actin cytoskeletal rearrangement to force generation evoked by PKC activation in resistance arteries. (CIHR MOP‐97988, CIHR & AHFMR Fellowships)
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".