Notch2 expression in association with pancreatic cancer stem cells
Bibliographic record
Abstract
Objective: To isolate and identify the pancreatic cancer stem cells based on surface marker Notch2. Methods: The Notch2＋ cells in pancreatic cancer Bxpc-3 and Panc-1 cells were separated by magnetic activated cell sorting (MACS) technique. The tumorigenic potent of these cells was evaluated via transplantation of the cells into NOD-SCID mice. Notch2＋ cells were cultured with serum-free medium to detect the tumorsphere formation ability. The expressions of stem cell-related markers—octamer-binding transcription factor 4 (Oct4), Nanog and pancreatic stem cell marker pancreatic and duodenal homeobox 1 (PDX1) in the isolated cells were measured by immunofluorescent staining. The K-ras mutation was detected by DNA sequencing technology. Results: The Notch2＋ cells possessed a markedly tumorigenic ability. The positive expressions of core transcription factors (Oct4, Nanog and PDX1) of embryonic stem cells were observed in these Notch2＋ cells. The Notch2＋ cells were able to form spheres when cultured in serum-free medium. K-ras mutations were found in these cells. Conclusion: The Notch2＋ cells in human pancreatic cancer Bxpc-3 and Panc-1 cells exhibit the properties of cancer stem cells. DOI:10.3781/j.issn.1000-7431.2011.08.006
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".