Bibliographic record
Abstract
R-GECO1 is a red-shifted calcium indicator (Zhao et al. 2011). Besides being suitable for monitoring the activity of identified GFP-labeled neurons, it could also be useful for optogenetic neural circuit mapping where Chr2 expressing neurons can be activated using blue-light. To apply this new tool in <em>Drosophila</em> neurobiology we have made a UAS-R-GECO1 transgenic stock so that R-GECO1 can be expressed in many different parts of its nervous system using the Gal4/UAS system (Brand and Perrimon 1993). <strong>A.</strong> The coding sequence of R-GECO1 was first PCR amplified from the CMV-R-GECO1 plasmid (donated by Robert Campbell, Univ. of Alberta, Edmonton, Canada) using the following oligos:1.FWD: 5'-GAGAGGTACCAATCAAAATGGTCGACTCTTCACGTCGTAAGTG-3' and REV: 5'-GAGAGGATCCTACTTCGCTGTCATCATTTGTACAAAC-3'. This PCR product [KpnI-AATCAAA-R-GECO-BamHI, ~1.2 kb] was digested with KpnI + BamHI restriction enzymes and subcloned into the KpnI & BamHI sites of the pJFRC14-MCS-attB vector (modified from Pfeiffer et al. 2010). This transgene was targeted for insertion into the <em>Drosophila</em> second chromosome (attP40 site) using the PhiC31 integrase (microinjection by BestGene Inc.). <strong>B. </strong>The expression of R-GECO1 in the larval CNS (middle panel). Flies containing the UAS-R-GECO1 transgene were crossed to the Cha-Gal4, UAS-GFP recombinant line. The CNS of progeny larvae were visualized using an epifluoresence microscope with appropriate optical filter settings. Arrows indicate fluorescence in cell bodies. Br: Brain, VC: ventral cord. <strong>References:</strong> 1. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Brand AH, Perrimon N. Development. 1993:401-15. PMID: 8223268 2. Refinement of tools for targeted gene expression in <em>Drosophila</em>. Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, Rubin GM. Genetics. 2010.186:735-55. PMID: 20697123 3. An expanded palette of genetically encoded Ca²⁺ indicators. Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE. Science. 2011.1888-91. PMID: 21903779 <strong>Acknowledgements: </strong>Many thanks to the Macleod lab (University of Texas Health Science Center at San Antonio, USA) and the Louis Lab (CRG, Barcelona, Spain) for the excellent support and discussions.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.011 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".