Arachidonate distribution in resting and proliferating primary human T cells
Bibliographic record
Abstract
Arachidonate‐phospholipid remodelling is characterised by the transfer of arachidonate (AA) between cellular phosphatidylcholine (PC) and phosphatidylethanolamine (PE) species. It has been suggested that AA‐phospholipid remodelling may control cellular AA distribution and availability. In this study, the cellular distribution of AA was measured in resting and in anti‐CD3+IL‐2 ‐stimulated primary human T cells. In pulse‐chase experiments a very rapid redistribution of [ 3 H]AA from PC to PE was measured in stimulated proliferating cells compared to resting cells. This increased remodelling was accompanied by a marked shift in the mass distribution of AA into 1‐alkyl‐ and 1‐alk‐1‐enyl‐linked PE species in stimulated cells. Minor differences in AA distribution were measured in cells arrested in different phases of the cell cycle (G1/S, G2/M and S), with the most marked changes observed when the cells transitioned from G0 into the cell cycle. The re‐distribution of AA in proliferating T cells was reversed when the stimulus was removed and the cells ceased to proliferate. Previous studies linked the expression of group IVC phospholipase A 2 with a redistribution of AA into PE species suggesting a role for this enzyme in remodelling. However, remodelling activity was not modified in HEK293 cells overexpressing this enzyme, suggesting that a different isozyme is likely involved. Microarray experiments are now underway with the aim of identifying which enzymes are possibly involved in the remodelling of arachidonate in cycling human T cells. (Supported by the Canadian Institutes of Health Research and the Canada Research Chairs Program).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".