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Record W2262877696 · doi:10.82308/33206

Characterization of a family of yeast transcriptional regulators : the zine cluster proteins

2003· dissertation· en· W2262877696 on OpenAlexfundno aff
Bassel Akache

Bibliographic record

VenueeScholarship@McGill (McGill) · 2003
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicFungal and yeast genetics research
Canadian institutionsnot available
FundersMedical Research CouncilTakeda OncologyMedical Research Council CanadaMcGill University
KeywordsBiologyGeneGeneticsSaccharomyces cerevisiaeMultiple drug resistanceGene clusterZinc fingerTranscriptional regulationGenomeCell biologyComputational biologyGene expressionTranscription factorDrug resistance

Abstract

fetched live from OpenAlex

Members of the zinc cluster (or binuclear cluster) protein family are characterized by a Zn(II)Cys6 zinc finger involved in DNA recognition and binding. These fungal proteins are transcriptional regulators of genes involved in a wide variety of cellular processes. One member, Gal4p, is involved in galactose metabolism, while others play a major role in primary and secondary metabolism, control of meiosis, and multidrug resistance. Sequencing of the Saccharomyces cerevisiae genome has revealed that 55 genes encoding putative zinc cluster proteins are present in budding yeast. However, the roles of many of these zinc cluster proteins are unknown. In order to better understand their functions, we have performed a phenotypic analysis of these putative zinc cluster proteins. We have implicated a number of them in a variety of processes in the cell, including multidrug resistance. Stb5p has been shown to be a major player in regulating the expression of multidrug resistance genes. Other zinc cluster activators of multidrug resistance genes include Pdr1p, Pdr3p, and Yrr1p. These regulators of multidrug resistance appear to interact with each other, forming many different sub-populations of homo- and heterodimers. Stb5p is found predominantly as a heterodimer with Pdr1p. It also appears that Pdr1p is a master regulator able to interact with many different partners, enabling it to mediate control over multidrug resistance genes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.243
Teacher spread0.229 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2003
Admission routes1
Has abstractyes

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