Modulation of the human paraoxonase gene family members by various effectors in Caco‐2 cells
Bibliographic record
Abstract
The human paraoxonase (PON) gene family is composed of three members (PON1, PON2, PON3) aligned next to each other on chromosome 7. They are highly conserved in mammals, display similarities in their structural properties (~65% identity atthe amino acid level) and seem to play an important part in oxidative stress. Recently, PONs have been detected in the gastrointestinal tract, but their physiological roles and regulation remain obscure. Our objective was to examine the modulation of PON1, PON2 and PON3 in the Caco‐2 cell line. Fe 2+ ‐ascorbate‐mediated lipid peroxidation promoted MDA formation without affecting sucrase activity, transepithelial resistance, DNA and protein content, as well as cell viability. The addition of the oxygen radical‐generating system reduced the gene expression of PON1, PON2 and PON3. Pre‐incubation with the antioxidant BHT or Trolox restrained the production of MDA and the inhibitory effect on PON mRNA levels. Similarly, the administration of CAPE, an inhibitor of NF‐kB activity, reversed the negative influence of inflammatory agents, including LPS, TNF‐α and INF‐γ. Rosiglitazone and DHA enhanced the gene expression of PON1 and PON3 without any influence on PON2 mRNA levels. Our data indicate that various effectors may impact on the gene expression of PON1, PON2 and PON3, sometimes with a distinct modulation. Supported by Crohn's and Colitis Foundation of Canada.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".