Expression of Jejunal Apical Excitatory Amino Acid Carrier 1 (EAAC1) is Maintained in Piglets with Bowel Inflammation
Bibliographic record
Abstract
The objective of this study was to examine jejunal excitatory amino acid carrier 1 (EAAC1) expression in response to bowel inflammation induced with dextran sodium sulfate (DSS) in the piglet model. Piglets at the age of d 5 were fed a commercial source of liquid formula, fitted with an intra‐gastric catheter and administrated with either 2.5 g of DSS/kg body weight (DSS, n = 5) in saline or saline (control, n = 6) in two injections daily for a period of 10 d prior to jejunal tissue harvesting. Small and large bowel inflammations were confirmed with representative pro‐inflammatory cytokine profiling. Predominant EAAC1 immuno‐reactive bands of 57 and 73 kDa were detected in the jejunal homogenate and the apical membrane (AM), respectively, consistent with the nonglycosylated pre‐mature and the glycosylated mature forms of the EAAC1 protein. Expression of the 57‐kDa pre‐mature EAAC1 protein in the jejunal homogenate was lower ( P < 0.05) in the DSS group compared with the control. However, there was no difference ( P > 0.05) in abundance of the 73‐kDa mature EAAC1 in AM between the two groups. Furthermore, there was no difference ( P > 0.05) in EAAC1 mRNA abundance between the DSS and the control groups. These results have suggested that expression of 73‐kDa mature EAAC1 protein in the gut apical membrane is maintained in piglets with during bowel inflammation. Supported by AMFNET of NSERC.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".