CFTR anion channel regulation by phosphorylation of focal adhesion kinase at tyrosine 407 in osmosensing mitochondria‐rich ion transporting cells of killifish ( <i>Fundulus heteroclitus</i> )
Bibliographic record
Abstract
Focal adhesion kinase (FAK) is a tyrosine kinase with multiple Y sites and an ezrin/radixin/moesin binding site. Antibodies to the phosphorylated form of FAK at Y407 and Y587 are colocalized with the Cl − channel CFTR in the apical membrane of mitochondria‐rich (MR) cells of seawater‐acclimated euryhaline killifish opercular epithelium. Hypotonic treatment (80%, 224 mOsm/kg) causes rapid, reversible cell swelling (observed via confocal microscopy of living cells in situ ), inhibition of Cl − secretion and dephosphorylation of FAK pY407 but no effect on FAK phosphorylated at Y576. Re‐phosphorylation of FAK at Y407 specifically in the apical membrane of MR cells was immunohistochemically detected following 10‐min treatments with forskolin (10 μM), 3‐isobutyl‐1‐methylxanthine (IBMX, 0.1 mM) and isoproterenol (1.0 μM), all known to increase cAMP and activate CFTR. There is parallel recovery of Cl − secretion by MR cells in the isolated epithelium, detected electrophysiologically. These results suggest involvement of phosphorylation of FAK pY407 but not pY576 in cell signaling pathways regulating the Cl − transport function of CFTR. (Supported by NSERC Canada)
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".