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Adiponectin and Leptin Affect Cell Cycle Regulation and Adiponectin Receptor Function

2009· article· en· W2270248045 on OpenAlexaff
Michael K. Connor, O’Llenecia S. Walker

Bibliographic record

VenueThe FASEB Journal · 2009
Typearticle
Languageen
FieldMedicine
TopicAdipokines, Inflammation, and Metabolic Diseases
Canadian institutionsYork University
Fundersnot available
KeywordsAMPKAdiponectinInternal medicineEndocrinologyAdiponectin receptor 1LeptinAdipokinePhosphorylationBiologyProtein kinase BChemistryCell biologyProtein kinase AMedicineInsulinInsulin resistanceObesity

Abstract

fetched live from OpenAlex

To establish a molecular link between obesity and cancer we examined the effects of the adipocyte produced peptides leptin (LEP) and adiponectin (ADIPO) on MCF7 cell cycle regulation. p27 levels decreased and increased with LEP and ADIPO, respectively, suggesting adipokine‐mediated MCF7 cell cycle regulation. LEP overcame the effects of ADIPO on p27 protein levels. LEP increased adiponectin receptor (ADIPOR) levels despite inhibiting ADIPO action, suggesting that LEP is stabilizing and inactivating ADIPOR. Inhibition of AKT enhanced ADIPOR protein levels, increased 14‐3‐3/ADIPOR binding and inhibited AMPK phosphorylation. Thus, phosphorylation of ADIPOR by AKT disrupts ADIPOR/14‐3‐3 interactions and promotes ADIPOR degradation. ADIPO induces AMPK‐mediated phosphorylation of p27 at T198 and stabilizes p27 protein, effects similar to those seen after AMPK activation by AICAR. ADIPO and AICAR effects on p27 phosphorylation were inhibited by the AMPK inhibitor compound C. ADIPO also induced decreases in ADIPOR protein levels. Our data suggest that ADIPO and LEP exert antagonistic effects on mammary cell cycle regulation, in part by regulating the function and expression of ADIPOR.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.238
Teacher spread0.228 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes1
Has abstractyes

Explore more

Same venueThe FASEB Journal→Same topicAdipokines, Inflammation, and Metabolic Diseases→French-language works237,207→