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YJL185c encodes a peroxisomal protein that binds Inp1p and is required for peroxisome retention

2008· article· en· W2273910505 on OpenAlexaff
Monica Fagarasanu, Fred D. Mast, Richard A. Rachubinski

Bibliographic record

VenueThe FASEB Journal · 2008
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPeroxisome Proliferator-Activated Receptors
Canadian institutionsUniversity of Alberta
Fundersnot available
KeywordsPeroxisomeOrganelleCell biologySaccharomyces cerevisiaeBiologyProtein targetingCell divisionCompartmentalization (fire protection)Green fluorescent proteinYeastBiochemistryGeneCellMembrane proteinEnzyme

Abstract

fetched live from OpenAlex

The inheritance of cellular organelles from mother cell to daughter cell is critical for eukaryotic cells to maintain the metabolic benefits of compartmentalization. Because of its asymetrical division, the budding yeast Saccharomyces cerevisiae has been used extensively to understand the molecular pathways involved in the inheritance of different organelles. The inheritance of peroxisomes is dependent on the peroxisomal protein Inp1p, which is directly involved in tethering peroxisomes to anchoring structures at the cell cortex. Although Inp1p is a major factor acting in the peroxisomal retention pathway, we proposed that its action in peroxisome retention is regulated by additional proteins. with which it interacts. A protein of unknown function encoded by the open reading frame YJL185c was shown to interact with Inp1p by the yeast two‐hybrid system. Cells deleted for YJL185c exhibit an altered peroxisome morphology and a preferred localization of peroxisomes to the bud neck. Yjl185p tagged with green fluorescent protein, GFP, shows both a peroxisomal and a weak cortical localization. Our studies help to clarify the mechanism of action of Inp1p in peroxisome inheritance and identify Yjl185p as a novel protein involved in peroxisome inheritance.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.032
GPT teacher head0.249
Teacher spread0.216 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2008
Admission routes1
Has abstractyes

Explore more

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