PALMITOYLATION OF G‐PROTEIN ALPHAQ IN HYPOXIC PULMONARY HYPERTENSION
Bibliographic record
Abstract
Introduction Hyperreactivity of thromboxane prostanoind receptor (TP) is a major contributor to persistent pulmonary hypertension of the newborn (PPHN). We previously reported that in hypoxic pulmonary artery smooth muscle cells (PASMCs) TP couples mainly with G‐protein alphaq (Gαq), which undergoes palmitoylation. Palmitoylation is a dynamic, reversible post‐translational modification of the N‐terminal cysteine residues of Gαq, which is required for efficient receptor‐Gαq interaction. Objective To study the role of Gαq palmitoylation in hypoxic pulmonary hypertension. Methods PPHN was induced in newborn piglets by exposure to normobaric hypoxia (FiO 2 0.10) for 72h; age‐matched normoxic piglets served as controls. Serum deprived primary cultured myocytes from newborn pig (day 0) were placed in hypoxic (10% O 2 ) or normoxic (21% O 2 ) environment for 72h. Palmitoylable cysteine to alanine mutants of Gαq were obtained commercially and transiently transfected in HEK293T cells stably expressing TP. Calcium mobilization to TP stimulation was studied by calcium indicator dyes in hypoxic and normoxic conditions. Metabolic labeling of Gαq by 3 H palmitic acid is currently pursued under hypoxia and normoxia. Results Pretreatment with 2‐BrP decreased the maximum contractile response and calcium mobilization of hypoxic pulmonary artery rings and PASMCs respectively. 2‐BrP had minimal effect on rings and PASMCs from normoxic piglets. Conclusions Palmitoylation of Gαq could play role in hypoxia‐induced hyperreactivity of TP. Funding: MHRC‐MICH Graduate studentship
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".