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TNFR1 functions as a survival receptor in TNFR2-/- CD8 T cells (50.19)

2010· article· en· W2281305531 on OpenAlexaffabout
Yuh‐Ching Twu, Hung‐Sia Teh

Bibliographic record

VenueThe Journal of Immunology · 2010
Typearticle
Languageen
FieldImmunology and Microbiology
TopicImmune Cell Function and Interaction
Canadian institutionsUniversity of British Columbia
Fundersnot available
KeywordsCD8Cytotoxic T cellCell biologyTumor necrosis factor alphaApoptosisTRAF2BiologyTransfectionMolecular biologyImmune systemCancer researchCell cultureImmunologyIn vitroTumor necrosis factor receptorBiochemistry

Abstract

fetched live from OpenAlex

Abstract TNF/TNFR superfamily members play central roles in host defense, inflammation, apoptosis, autoimmunity and organogenesis. Previous reports showed that TNFR2 regulates T cell activation by lowering the activation threshold and providing costimulatory signaling. Furthermore, activated TNFR2-/- CD8 cells are highly resistant to AICD. Here, we show that blocking of activated wild-type (wt) CD8 cells with anti-TNFR2 antibodies also renders these cells resistant to AICD. The resistance of activated TNFR2-/- CD8 cells to AICD correlates with the accumulation of TRAF2. Retroviral transfection studies showed that TRAF2 overexpression was partially effective in preventing AICD in activated wt CD8+ cells. Furthermore, neutralization of TNF-α inhibits the proliferation of anti-CD3-stimulated wt CD8 T cells and increases apoptotic death cells in activated TNFR2-/- cells. TNF-α blocking also reduced TRAF2 accumulation in activated TNFR2-/- CD8 cells, which correlates with their increase susceptibility to AICD. AICD-resistant TNFR2-/- CD8 cells expressed elevated levels of phosphorylated IκBα and neutralization of TNF-α blocked this increase. These results indicate that in activated TNFR2-/- CD8 cells, TNFR1 functions as a survival receptor by promoting TNF-α-mediated regulation of TRAF2 levels and the extent of phosphorylation of pro-survival signaling molecules such as IκBα. This work is supported by the Canadian Cancer Society.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.014

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0040.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.237
Teacher spread0.226 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2010
Admission routes2
Has abstractyes

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