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Identification CD79a and CD79b homologs in channel catfish, Ictaulurus punctatus

2008· article· en· W2281819587 on OpenAlexaff
Manoranjan Sahoo, Eva‐Stina Edholm, James L. Stafford, Eva Bengtén, Norman W. Miller, Melanie Wilson

Bibliographic record

VenueThe FASEB Journal · 2008
Typearticle
Languageen
FieldImmunology and Microbiology
TopicT-cell and B-cell Immunology
Canadian institutionsUniversity of Alberta
FundersNational Institutes of Health
KeywordsCatfishBiologyMolecular biologyImmunoglobulin domainTransmembrane domainTransmembrane proteinImmunoreceptor tyrosine-based activation motifEpitopeAntibodyGeneTransfectionImmunoprecipitationSignal peptideCell biologyTyrosine kinaseReceptorPeptide sequenceSignal transductionGeneticsSH2 domain

Abstract

fetched live from OpenAlex

The catfish (Ip) B cell receptor accessory molecules CD79a and CD79b homologs have been identified. Each are encoded by single copy genes that encode proteins containing a signal peptide, an extracellular immunoglobulin domain, a transmembrane region and a cytoplasmic tail containing an immunoreceptor tyrosine kinase activation motif (ITAM). At the message level, both IpCD79a and IpCD79b correlate well with IgM message expression since they are highly expressed in membrane IgM + peripheral blood leukocytes (PBL) and in catfish clonal B cell lines, but not in IgM − PBL or in clonal T cells or macrophages. Such findings indicate that IpCD79a and IpCD79b expression is B cell restricted. Furthermore, studies using catfish clonal B cells (3B11) transfected with constructs encoding epitope‐tagged IpCD79 proteins revealed that IpCD79a is expressed as a 45 kDa protein and IpCD79b is expressed as a 32 kDa protein. These correlate with the molecular weights of putative tyrosine phosphorylated accessory proteins that are co‐immunoprecipitated with surface IgM following anti‐Ig stimulation. Supported by NIH grant R01 AI19530 .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.216
Teacher spread0.200 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2008
Admission routes1
Has abstractyes

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