Molecular cloning and expression of a jasmonate biosynthetic gene allene oxide cyclase from <i>Camellia sinensis</i>
Bibliographic record
Abstract
As a family of signaling plant hormone, jasmonic acid plays an important role in coordinating plant defense responses to pests and pathogen attack through transcriptional and metabolic changes. In the jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC) is an essential enzyme. Here we cloned a cDNA from tea plant (Camellia sinensis), named as CsAOC (GenBank: HQ889679), which was 916 bp, containing an open reading frame (738 bp) encoding 245 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsAOC was highly homologous to AOC from other plant species. Phylogenetic analysis indicated that CsAOC was clustered in a closely related subgroup with AOC of Ipomoea nil. The full-length coding region of CsAOC was ligated with pET-32a and successfully expressed in E. coli BL21 (DE3), and purified. Real-time quantitative PCR analysis revealed that methyl jasmonate (MeJA) treatment on its own potently enhanced its expression over the control (healthy leaves), suggesting feedback activation of CsAOC. The expression under salicylic acid (SA) and wounding treatments was up-regulated. The mRNA expression of CsAOC could be induced by tea geometrids and tea green leafhoppers.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".