Interaction of Selective Amino Acid Residues of K<sub>Ca</sub> Channels with Carbon Monoxide
Bibliographic record
Abstract
The activation of big-conductance K(Ca) channels in vascular smooth muscle cells by carbon monoxide (CO) has been demonstrated previously. One specific target of CO on K(Ca) channel proteins is the histidine residue. The roles of other amino acid residues on the functionality of K(Ca) channels, as well as their reactions to CO, have been unclear. In the present study, the cell-free single channel recording technique was used to investigate the chemical modification of K(Ca) channels by CO and other chemical agents. The modification of negatively charged carboxyl groups and the epsilon -amino group of lysine did not affect the open probability, but decreased single-channel conductance of K(Ca) channels. When sulfhydryl groups of cysteine were modified with N-ethylmaleimide, the open probability of K(Ca) channels was decreased, but single-channel conductance was not affected. None of the above chemical modifications affected the CO-induced increase in the open probability of K(Ca) channels. However, N-ethylmaleimide treatment reduced the stimulatory effect of nitric oxide (NO) on K(Ca) channels. Finally, pretreatment of smooth muscle cells with NO abolished the effects of subsequently applied CO on K(Ca) channel proteins. Our study demonstrates that CO and NO acted on different amino acid residues of K(Ca) channel proteins. The interaction of CO and NO determines the functional status of K(Ca) channels in vascular smooth muscle cells
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".