Exploring the Mechanism of Acyl Carrier Protein Interactions and Acyl Transfer
Bibliographic record
Abstract
As an essential protein in bacterial fatty acid synthesis, acyl carrier protein (ACP) is an attractive anti‐microbial drug target. The three‐dimensional structure of ACP is comprised of three main parallel α–helices, which create a hydrophobic binding pocket that encloses a fatty acyl chain when attached. ACP interacts with most partner enzymes through the acidic helix II, but the exact mechanism of interaction and acyl transfer to a partner enzyme is poorly understood. For acyl transfer to occur, we hypothesize that a conformational change is required. To examine this requirement, we have analyzed a cyclic version of Vibrio harveyi ACP created by split‐intein technology (Volkmann, et al . (2010) JBC 285 : 8605) using molecular dynamics, mass spectrometry, tryptophan fluorescence, and other biophysical techniques. We have demonstrated that cyclic ACP is more thermodynamically stable than wild‐type linear ACP. Furthermore, we show that cyclic ACP is able to act as a substrate for holo‐ACP synthase, yielding a cyclic ACP capable of carrying fatty acids. (Supported by NSERC and an NSHRF Student Research Award)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".