Heat Shock Protein Concentration and Clarity of Porcine Lenses Incubated at Elevated Temperatures
Bibliographic record
Abstract
Purpose: to quantify the concentration of heat shock proteins in lenses and culture medium in organ culture at elevated temperatures. Methods: Pig lenses were dissected aseptically and incubated in medium M199 without serum for 4 days and those with protein leakage less than 10 mg/L were taken for heat shock (HS). HS was performed by incubation for 1 hr at temperatures from 37°C to 55°C. After incubation for 24 hr, the lenses were weighed and homogenized and the medium was collected. The medium and lens homogenates were analysed for Hsp 70 and Hsp 27 by western blotting. ScantoxTM and analysis of lens photographs were used to estimate cataract focal length variability. Results: Hsp 70 was generally increased in the lens at temperatures above 39°C but the response was diminished at 55°C. No changes in Hsp27 concentration in the lens were observed. Interestingly, Hsp70 levels also increased in the culture medium after HS at 42.5°C. Increased temperatures were also associated with greater focal length variability and with loss of lens weight during the 24 hours following HS. Conclusions: Hsp70 concentration in the lens, its release, and the focusing ability of the lens were altered in response to HS The observation that Hsp70 is released by the stressed lens may suggest an important paracrine function, given the potential signaling properties of these proteins. Supported by CSA and NSERC 8170‐05 RGPIN
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".