In Situ Measurement of Oligomerization State of NBCe1‐A in Kidney Tissues using Spatial Fluorescence Intensity Fluctuation Analysis
Bibliographic record
Abstract
NBCe1‐A plays an important role in absorbing sodium bicarbonate across the basolateral membrane of the proximal tubule. We have previously showed that minimal functional unit of NBCe1‐A is a monomer, and based on in‐vitro biochemical studies in HEK293 cells, the oligomeric state of the cotransporter was shown to be predominantly dimeric with monomeric and higher oligomeric forms also present. In the present study, we developed an in situ measurement methodology to determine the oligomeric state of NBCe1‐A without requiring tissue disruption. We used fluorescent moment image analysis and spatial intensity distribution analysis (SpIDA) to study the oligomeric state of NBCe1‐A. Both methods allow for unbiased quantitative measurement of fluorescent particle densities and oligomerization states within individual images acquired with laser‐scanning microscopy. Initially we examined basal membranes of highly adherent CHO K1 cells expressing eGFP‐tagged NBCe1‐A, and showed that NBCe1‐A existed primarily in the monomeric state and negligibly in the dimeric state on the cell membrane. We immunolabeled NBCe1‐A in kidney tissues demonstrating the NBCe1‐A is present in dimeric and rarely in higher order oligomeric states. As a control, we compared the results obtained from LSM images of tissues of NBCe1‐A knockout mouse. These experiments demonstrate for the first time the in situ oligomeric state(s) of NBCe1‐A.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".