Bibliographic record
Abstract
Np95 (also known as Uhrf1) is a proliferation‐associated E3 ubiquitin ligase that participates in maintenance of DNA methylation and histone H3 lysine 9 trimethylation trimeH3K9) associated with heterochromatin. Although cells deficient in Np95 have previously been shown to exhibit greater sensitivity to DNA damaging agents, the role of this protein in the cellular response to DNA damage is unknown. Here we report that Np95 is critical for the maintenance of genome integrity. In the absence or presence of DNA damaging agents, Np95 deficiency leads to an increase in the number of cells with chromosomal instability and elevated levels of γH2AX, a cellular marker that signifies the presence of DNA double strand breaks. Although H2AX status has previously been shown to impact checkpoint activation, Np95 deficient cells exhibit intact checkpoint activation compared to wild‐type cells. Furthermore, DNA damage signalling pathways that activate Chk2 and FancD2 are intact in Np95 deficient cells. Loss of Np95 results in a redistribution of focal trimeH3K9 staining to the nuclear periphery. We observed that (ii) focal staining of heterochromatin protein HP1β but not HP1α dissipates following DNA damage and (ii) this loss of focal HP1β staining is accentuated in Np95 deficient cells. Our findings indicate that maintenance of heterochromatin by Np95 is crucial for genome integrity and protection from DNA damage.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".