Mechanistic studies on UDP‐Galactopyranose mutases from Aspergillus fumigatus and Trypanosoma cruzi
Bibliographic record
Abstract
UDP‐Galactopyranose mutase (UGM) is a flavoprotein that catalyzes the conversion of UDP‐galactopyranose to UDP‐galactofuranose. UGM serves as the sole biosynthetic source of galactofuranose, which is found on the cell surface of many bacteria, fungi, and parasites. UGM is a unique flavoprotein since it requires the flavin to be reduced for activity, yet no net redox change occurs during catalysis. Currently, the exact mechanism of isomerization is unknown. UGM is also of interest because it is a novel drug target for several diseases due to the importance of galactofuranose for the survival of M. tuberculosis and for virulence of the fungus, A. fumigatus, and the human parasites, such as L. major and T. cruzi . While many studies have been done on the prokaryotic UGM, little is known about eukaryotic UGM. We present, for the first time, the recombinant expression, purification, and characterization of eukaryotic UGMs from A. fumigatus and T. cruzi . We also report limited proteolysis, steady‐state kinetics, rapid‐reaction kinetics, and site‐directed mutagenesis data that demonstrate significant differences between prokaryotic and eukaryotic UGMs.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".