Impaired anaplerosis in VLCAD (very long chain acyl‐CoA dehydrogenase) deficient mouse hearts
Bibliographic record
Abstract
Current evidence suggests the benefit of a diet containing anaplerotic odd‐chain medium chain fatty acid (MCFAs) for cardiac symptoms in patients with long chain fatty acid oxidation defects (Roe & coll. JCI, 110(2):259‐69, 2002), although the underlying mechanism remains unclear. We conducted a study to evaluate the anaplerotic status of VLCAD −/− mouse hearts, using our working mouse heart model and 13 C‐methodology. Furthermore, the expression of selected anaplerotic genes (using real time PCR), and the myocardial levels of CAC intermediates were assessed in fed and 24h fasted mice. Compared to controls VLCAD +/+ , 3 month‐old VLCAD −/− hearts showed a 40% lower anaplertoic flux through pyruvate carboxylation (p<0.05). Furthermore, VLCAD −/− hearts displayed lower mRNA levels for enzymes involved in anaplerosis, namely pyruvate and propionyl‐CoA carboxylase, but not malic enzyme, when compared to their normal counterparts, changes that were however age‐ and condition‐dependent. Besides, two‐way Anova revealed a species effect on CAC intermediates levels, specifically succinate and malate, which were lower in VLCAD −/− hearts from 7 month‐old mice. Collectively, our data highlight, impaired anaplerosis in VLCAD −/− mouse hearts, suggesting a potential beneficial role for odd‐chain MCFAs. (Supported by NIH & CIHR)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".